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hil 7  (R&D Systems)


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    Structured Review

    R&D Systems hil 7
    Hil 7, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+7/Recombinant+Mouse+IL-7+Protein/pmc13059316-49-29-30
    Average 95 stars, based on 136 article reviews
    hil 7 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Cell type-specific enhancers regulate IL-22 expression in innate and adaptive lymphoid cells
    Article Snippet: EL4 and 63-12 cells were cultured in RPMI (Gibco) supplemented with 10% fetal bovine serum (Sigma), 1X Penicillin-Streptomycin (Gibco), and 50 μM β-mercaptoethanol (Sigma). .. Mnk3 cells, described previously , were cultured in DMEM supplemented with 10% fetal bovine serum, 1X Penicillin-Streptomycin, 50 μM β-mercaptoethanol, 10 ng/ml mouse IL-7 (R&D Systems, 407- ML/CF), and 10 ng/ml mouse IL-2 (R&D Systems, 402-ML/CF). .. HEK293T cells were maintained in DMEM (Gibco) supplemented with 10% fetal bovine serum, 1X Penicillin-Streptomycin, and 50 μM β-mercaptoethanol.

    Article Title: AMG 509 (Xaluritamig), an Anti-STEAP1 XmAb 2+1 T-cell Redirecting Immune Therapy with Avidity-Dependent Activity against Prostate Cancer
    Article Snippet: JANUARY 2024 CANCER DISCOVERY | 91

    Article Title: Cell type-specific enhancers regulate IL-22 expression in innate and adaptive type 3 lymphoid cells.
    Article Snippet: EL4 and 63-12 cells were cultured in RPMI (Gibco, 61870-036) supplemented with 10% fetal bovine serum (Sigma, F4135), 1X Penicillin-Streptomycin (Gibco, 15140-122), and 50 μM β-mercaptoethanol (Sigma, M3148). .. Mnk3 cells25, were cultured in DMEM (Gibco, 11995-065) supplemented with 10% fetal bovine serum, 1X Penicillin-Streptomycin, 50 μM β-mercaptoethanol, 10 ng/ml mouse IL-7 (R&D Systems, 407-ML/CF), and 10 ng/ml mouse IL-2 (R&D Systems, 402-ML/CF). ..

    Activation Assay:

    Article Title: AMG 509 (Xaluritamig), an Anti-STEAP1 XmAb 2+1 T-cell Redirecting Immune Therapy with Avidity-Dependent Activity against Prostate Cancer
    Article Snippet: JANUARY 2024 CANCER DISCOVERY | 91

    Purification:

    Article Title: Th1-poised naive CD4 T cell subpopulation reflects anti-tumor immunity and autoimmune disease
    Article Snippet: Then using the enriched CD4 + T cells, naive CD4 + T cells (TCR-β + , CD4 + , CD25 - , CD62L hi , CD44 low , IL-7R sup-hi or low ) were sorted using FACS (ARIA Fusion, BD Bioscience). .. Purified naive CD4 + T cells were seeded in 96-well-plate in 2 × 10 5 cells/well and stimulated by plate-bound anti-mouse CD3/CD28 (5 μg/ml, BD bioscience) and/or mouse IL-7 (10 ng/ml, R&D systems), IL-12 (20 ng/ml, peprotech), IL-18 (20 ng/ml, R&D systems) as indicated in Fig. . For Fig. , thymocytes were stimulated with the following cytokine conditions: mouse IFN-γ, mouse IL-12 (20 ng/ml, peprotech), mouse IL-27, mouse IFN-β, mouse IL-7, mouse IL-18, mouse IL-4, mouse IL-25, mouse IL-33, mouse TSLP, mouse IL-6, mouse IL-1β, mouse IL-23, mouse IL-2 (20 ng/ml, R&D systems), TGF-β (10 ng/ml, R&D systems). .. For further intracellular cytokine staining, cultured cells were re-stimulated by PMA (P-1585; Sigma, 40 ng/ml) and ionomycin (I-0634; Sigma, 0.8μg/ml) in the precence of monensin (51-2092kz; BD Bioscience).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Early age–related atrophy of cutaneous lymph nodes precipitates an early functional decline in skin immunity in mice with aging
    Article Snippet: .. The IL-7, CCL19, and CCL21 were quantified as described previously (1) using mouse IL-7, mouse CCL21, and mouse CCL19 DuoSet ELISA (R&D Systems) as per manufacturer’s instructions. .. The IL-7, CCL19, and CCL21 levels were normalized to total protein concentration of the homogenates determined using Pierce BCA protein Assay kit (ThermoFisher).

    other:

    Article Title: Th1-poised naive CD4 T cell subpopulation reflects anti-tumor immunity and autoimmune disease.
    Article Snippet: For further intracellular cytokine staining, cultured cells were re-stimulated by PMA (P-1585; Sigma, 40 ng/ml) and ionomycin (I-0634; Sigma, 0.8μg/ml) in the precence ofmonensin (51-2092kz; BD Bioscience).

    Article Title: Th1-poised naive CD4 T cell subpopulation reflects anti-tumor immunity and autoimmune disease.
    Article Snippet: Purified 2d2 (Vβ11+, CD4+, CD25-, CD62Lhi, CD44low, IL-7Rsup-hior low), OT-II (Vα2+, CD4+, CD25-, CD62Lhi, CD44low, IL-7Rsup-hi or low), or WT naive CD4+ T cells were labeled with CellTrace Violet (CTV; #C34557, Thermofisher) using the providedprotocol.



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    Image Search Results


    (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured with IL-7 for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

    Journal: bioRxiv

    Article Title: Bone marrow B cell collapse promotes bone metastasis in breast cancer

    doi: 10.64898/2026.04.21.720007

    Figure Lengend Snippet: (A) Schematic representation of B cell lymphopoiesis and markers that define stages of B cell development. (B, C) Representative contour plots (B) and frequency (C) of bone marrow B220 + CD19 - CD43 + CD93 + cells in C57BL/6 mice with IC injection of PBS or E0771 cells at day 17. (D, E) Representative contour plots (D) and frequency (E) of bone marrow B220 + CD19 + CD43 + CD93 + IgM - pro-B/large pre-B cells in mice described in (B). (F-I) Representative contour plots (F) and frequency of bone marrow B220 + CD19 + CD43 - IgM - IgD - small pre-B cells (G), B220 + CD19 + CD43 - IgM + IgD - immature B cells (H), and B220 + CD19 + CD43 - IgM + IgD + mature B cells (I) in mice described in (B). (J-O) Absolute numbers of B220 + CD19 + B cells (J) and frequency of bone marrow B cell subsets (K-O) in C57BL/6 mice with IC injection of PBS or E0771 cells at day 7. (P, Q) Representative pictures (P) of colony formation unit (CFU) assay of pre-B cells and quantification of CFUs (Q) from bone marrow of mice described in (B). (R) Frequency of bone marrow B cell subsets from bone marrow of mice described in (B) cultured with IL-7 for 3 days, relative to day 0. Statistical analysis was performed using unpaired two-tailed Student’s t test (C, E, G-O, Q, R). Data are represented as means ± SEM. Each dot represents a mouse in (C, E, G-O, Q, R). ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001, ∗∗∗∗p ≤ 0.0001.

    Article Snippet: 7.5 x 10 5 cells/ml were cultured in IMDM media containing Glutamax (Gibco, 31980030) supplemented with 10% FBS, 1 × P/S and 5μM 2-Mercaptoethanol, and 10 ng/ml of recombinant mouse IL-7 (Invitrogen, RP-8664) in a humidified incubator at 37 °C and 5% CO 2 .

    Techniques: Injection, Colony-forming Unit Assay, Cell Culture, Two Tailed Test